Expression vector

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Expression Vector

An Expression Vector (pronunciation: /ɪkˈsprɛʃən ˈvɛktər/) is a type of plasmid that is used in genetic engineering to introduce a specific gene into a target cell.

Etymology

The term "Expression Vector" is derived from the English words "expression" and "vector". The former refers to the process of gene expression, while the latter is a term used in molecular biology to refer to a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell.

Function

Expression vectors are designed to ensure the expression of the gene of interest in the target organism. They contain regulatory sequences, such as promoters and enhancers, which control the transcription and translation of the gene.

Types

There are several types of expression vectors, including bacterial expression vectors, yeast expression vectors, mammalian expression vectors, and insect expression vectors. The choice of vector depends on the specific requirements of the experiment, such as the type of organism that will be used and the desired level of protein expression.

Related Terms

  • Plasmid: A small, circular piece of DNA that is used as a vector in genetic engineering.
  • Promoter: A DNA sequence that initiates gene transcription.
  • Enhancer: A DNA sequence that increases the rate of gene transcription.
  • Transcription: The process of copying a gene's DNA sequence into RNA.
  • Translation: The process of converting the information in RNA into a protein.

External links

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