Enzyme assay

Enzyme assay

An Enzyme assay (pronunciation: /ˈɛnzaɪm ˈæseɪ/) is a laboratory procedure that measures the activity of enzymes. This process is crucial in the field of biochemistry and molecular biology.

Etymology

The term "Enzyme assay" is derived from the Greek word "ἔνζυμον" (enzymon), meaning "leavened", and the French word "essai", meaning "trial" or "attempt".

Types of Enzyme Assays

There are two main types of enzyme assays: continuous assays and discontinuous assays. Continuous assays measure the enzyme activity continuously, while discontinuous assays measure the enzyme activity at specific intervals.

Continuous Assays

In a continuous assay, the change in the amount of substrate or product is measured over time. This type of assay is often used in enzyme kinetics studies.

Discontinuous Assays

Discontinuous assays, on the other hand, involve stopping the reaction at specific time points and then measuring the amount of substrate or product. This type of assay is often used when the reaction rate is slow or when the substrate or product is unstable.

Related Terms

• Enzyme kinetics
• Enzyme inhibition
• Enzyme activator
• Enzyme substrate
• Enzyme product

See Also

• Biochemistry
• Molecular biology
• Enzymology

External links

• Medical encyclopedia article on Enzyme assay
• Wikipedia's article - Enzyme assay

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