Base sequence analysis
Base Sequence Analysis
Base sequence analysis (pronunciation: /beɪs 'siːkwəns ə'nælɪsɪs/) is a method used in molecular biology to determine the order of the four chemical building blocks - called "bases" - that make up the DNA molecule.
Etymology
The term "base sequence analysis" is derived from the English words "base" referring to the basic building blocks of DNA, "sequence" meaning the order in which something is arranged, and "analysis" referring to the detailed examination of the elements or structure of something.
Process
The process of base sequence analysis involves several steps. First, the DNA is isolated and purified. Then, it is broken down into smaller fragments. These fragments are then sequenced using a method such as Sanger sequencing or next-generation sequencing. The resulting sequence data is then analyzed using bioinformatics tools to identify genes, determine their function, and compare sequences between different organisms.
Related Terms
- DNA sequencing: The process of determining the precise order of nucleotides within a DNA molecule.
- Genomics: The study of whole genomes of organisms, and incorporates elements from genetics.
- Bioinformatics: An interdisciplinary field that develops methods and software tools for understanding biological data, in particular when the data sets are large and complex.
- Molecular biology: The branch of biology that deals with the structure and function of the macromolecules essential to life.
Applications
Base sequence analysis has a wide range of applications in various fields such as genetics, forensic science, biotechnology, and medicine. It is used to identify genes, determine their function, and compare sequences between different organisms. In medicine, it is used for genetic testing, personalized medicine, and in the study of diseases at the molecular level.
External links
- Medical encyclopedia article on Base sequence analysis
- Wikipedia's article - Base sequence analysis
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