BRET
BRET (Bioluminescence Resonance Energy Transfer)
BRET (pronounced: /bret/), an acronym for Bioluminescence Resonance Energy Transfer, is a technique used in molecular biology to study protein interactions.
Etymology
The term BRET is derived from the words Bioluminescence and Resonance Energy Transfer. Bioluminescence refers to the production and emission of light by a living organism, while Resonance Energy Transfer is a mechanism describing energy transfer between two light-sensitive molecules.
Definition
BRET is a non-invasive technique used for monitoring protein-protein interactions in real-time. It involves the transfer of energy from a bioluminescent protein to a fluorescent protein when they are in close proximity. This technique is widely used in cell biology and biochemistry to study various biological processes at the molecular level.
Related Terms
- Bioluminescence: The production and emission of light by a living organism.
- Resonance Energy Transfer: A mechanism describing energy transfer between two light-sensitive molecules.
- Protein-Protein Interaction: The specific physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.
- Molecular Biology: The branch of biology that deals with the structure and function of the macromolecules essential to life.
- Cell Biology: A branch of biology that studies cells – their physiological properties, their structure, the organelles they contain, interactions with their environment, their life cycle, division and death.
- Biochemistry: The branch of science concerned with the chemical and physicochemical processes and substances that occur within living organisms.
External links
- Medical encyclopedia article on BRET
- Wikipedia's article - BRET
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